molecular beacons

Assay Instruments

A number of spectrofluorometric thermal cyclers and spectrofluorometric temperature-controlled plate readers that monitor the progress of amplification reactions (i.e., Polymerase Chain Reaction (PCR), Nucleic Acid Sequence Based Amplification (NASBA), Transcription Mediated Amplification (TMA), Rolling Circle Amplification (RCA) and Strand Displacement Amplification (SDA)) by monitoring changes in fluorescence are available and are compatible with molecular beacons. In addition, fluorospectrometers are available to monitor the fluorescence generation of molecular beacons with and without nucleic acid targets present.

              

Company

               

Model

           

On Line


Applied Biosystems


7300 & 7500


www.appliedbiosystems.com

Applied Biosystems

PRISM 7700 & 7900HT

www.appliedbiosystems.com

bioMerieux

NucliSens EasyQ

www.biomerieux.com

Bio-Rad

CFX96 & CFX384

www.bio-rad.com

Bio-Rad

Chromo 4

www.bio-rad.com

Bio-Rad

iCycler iQ 5

www.bio-rad.com

Bio-Rad

MiniOpticon

www.bio-rad.com

Cepheid

GeneXpert

www.cepheid.com

Cepheid

SmartCycler

www.cepheid.com

Corbett Research

Rotor-Gene 6000

www.corbettresearch.com

Idaho Technology

RapidCycler 2

www.idahotech.com

NanoDrop Technologies

ND-3300 Fluorospectrometer

www.nanodrop.com

Eppendorf

Mastercycler ep realplex

www.eppendorf.com

Roche Applied Science

LightCycler 1.5 and 2

www.roche-applied-science.com

Roche Applied Science

LightCycler 480

www.roche-applied-science.com

Stratagene

Mx3000P & Mx3005P

www.stratagene.com

 

 

 


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Recent Publications from our group


Catrina IE, Bayer LV, Yanez G, McLaughlin JM, Malaczek K, Bagaeva E, Marras SAE, and Bratu DP (2016) The temporally controlled expression of Drongo, the fruit fly homolog of AGFG1, is achieved in female germline cells via P-bodies and its localization requires functional Rab11. RNA Biol 13: 1117-1132.



Vargas DY, Kramer FR, Tyagi S, and Marras SAE. (2016) Multiplex real-time PCR assays that measure the abundance of extremely rare mutations associated with cancer. PLoS ONE 11, e0156546.


We describe the use of “SuperSelective” primers that enable the detection and quantitation of somatic mutations whose presence relates to cancer diagnosis, prognosis, and therapy, in real-time multiplex PCR assays that can potentially analyze rare DNA fragments present in blood samples (liquid biopsies), thereby providing information that can be used to modify therapy for individual patients, prolonging (and improving the quality of) life.

Follow this link to access this article.