molecular beacons

Assay Instruments

A number of spectrofluorometric thermal cyclers and spectrofluorometric temperature-controlled plate readers that monitor the progress of amplification reactions (i.e., Polymerase Chain Reaction (PCR), Nucleic Acid Sequence Based Amplification (NASBA), Transcription Mediated Amplification (TMA), Rolling Circle Amplification (RCA) and Strand Displacement Amplification (SDA)) by monitoring changes in fluorescence are available and are compatible with molecular beacons. In addition, fluorospectrometers are available to monitor the fluorescence generation of molecular beacons with and without nucleic acid targets present.






On Line

Applied Biosystems

7300 & 7500

Applied Biosystems

PRISM 7700 & 7900HT


NucliSens EasyQ


CFX96 & CFX384


Chromo 4


iCycler iQ 5







Corbett Research

Rotor-Gene 6000

Idaho Technology

RapidCycler 2

NanoDrop Technologies

ND-3300 Fluorospectrometer


Mastercycler ep realplex

Roche Applied Science

LightCycler 1.5 and 2

Roche Applied Science

LightCycler 480


Mx3000P & Mx3005P




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Recent Publications from our group

Schlachter S, Chan K, Marras SAE, and Parveen N (2017) Detection and differentiation of lyme spirochetes and other tick-borne pathogens from blood using real-time PCR with molecular beacons. Methods in Molecular Biology 1616: 155-170.

Catrina IE, Bayer LV, Yanez G, McLaughlin JM, Malaczek K, Bagaeva E, Marras SAE, and Bratu DP (2016) The temporally controlled expression of Drongo, the fruit fly homolog of AGFG1, is achieved in female germline cells via P-bodies and its localization requires functional Rab11. RNA Biol 13: 1117-1132.

Vargas DY, Kramer FR, Tyagi S, and Marras SAE. (2016) Multiplex real-time PCR assays that measure the abundance of extremely rare mutations associated with cancer. PLoS ONE 11, e0156546.

We describe the use of “SuperSelective” primers that enable the detection and quantitation of somatic mutations whose presence relates to cancer diagnosis, prognosis, and therapy, in real-time multiplex PCR assays that can potentially analyze rare DNA fragments present in blood samples (liquid biopsies), thereby providing information that can be used to modify therapy for individual patients, prolonging (and improving the quality of) life.

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